AKT, also known as protein kinase B (PKB), plays a critical role in cell survival and apoptosis. AKT signaling is particularly important for transducing growth-factor mediated signaling, and regulating apoptotic signaling molecules by phosphorylating and inactivating several pro-apoptoptic proteins, including BAD and caspase-9. Disregulated AKT signaling can result in loss of apoptotic regulation, and so this pathway is a major target of anti-cancer research. Another important function of AKT is the regulation of glycogen synthesis through negative regulation of GSK-3α and β activity by phosphorylation.
TGR has developed several assays for the interrogation of these pathways:
AKT pathway targets
- phospho-caspase 9
- Total AKT
- phospho-p70S6 kinase