TGR has extensive knowledge of signaling characteristics of major pathways in many cell lines, and here we aim to provide a resource for scientists to optimize cellular experiments. However, if your cell line or pathway of interest is not listed here contact us to ask one our scientists.
Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are serine/threonine kinases that regulate cell cycle checkpoints in response to DNA damage. ATR is activated by a variety of agents that can induce DNA damage, including UV light, and particular chemicals that inhibit DNA replication or directly modify DNA, whereas ATM is induced primarily by conditions that induce double strand breaks in the DNA, such as ionizing radiation.
Upon induction, ATM/ATR regulate the activity of several diverse classes of protein, many of which have been directly implicated in cellular checkpoint signaling pathways. These include scaffolding proteins, regulatory proteins, and other kinases. Among the better understood ATM/ATR substrates are p53, Chk1 and Chk2. Located immediately downstream of ATM/ATR, parallel analysis of phosphorylation of a combination of Chk1, Chk2 and p53 provides an ideal platform for interrogating the induction of ATM/ATR-mediated signaling.
See below for cell-based applications on ATM/ATR signaling.
Click on the links below for cell-based applications for different signaling pathways and targets
JAK/STAT signaling pathway downloads
Akt signaling pathway downloads
MAPK signaling pathway downloads
NF-kappaB signaling pathway downloads
ATM/ATR signaling pathway downloads
mTOR signaling pathway downloads
TGFbeta signaling pathway downloads
Wnt signaling pathway downloads
Note: This list is regularly updated. Last update July 2011.