ELISA-One™ Targets

Monitoring cell signalling pathways accurately is essential for understanding many cellular processes and disease states. Phosphoproteins play a critical role in these processes and reliable sensitive measurement techniques are important to help further basic research and drug discovery research.

ELISA-ONE™ assay kits have been developed for key intracellular phosphoproteins (see list below), with each assay recognizing a specific phospho-epitope on each target. In addition, ELISA-ONE™ assay kits for each target, independent of phosphorylation status, are available for most targets for normalization.

ELISA-ONE™ assay kits are available as full 96-well assay kits, containing all components necessary for the assay.

 

ELISA-ONE™ Product List

Kit Description Part # Kit format
Test kits for measuring phospho-AKT 1/2/3 (S473) proteins ELK001 96-well kit
Test kits for measuring phospho-ERK 1/2 (T202/Y204) proteins ELK004 96-well kit
Test kits for measuring phospho-NF-κB p65 (S536) proteins ELK007 96-well kit
Test kits for measuring phospho-SMAD 1 (S463/465) proteins ELK010 96-well kit
Test kits for measuring phospho-STAT3 (Y705) proteins ELK013 96-well kit
Test kits for measuring phospho-STAT5 A/B (Y694/699) proteins ELK016 96-well kit
Test kits for measuring Total AKT 1/2/3 proteins ELK002 96-well kit
Test kits for measuring Total ERK 1/2 proteins ELK005 96-well kit
Test kits for measuring Total NF-κB p65 proteins ELK008 96-well kit
Test kits for measuring Total SMAD 1 proteins ELK011 96-well kit
Test kits for measuring Total STAT3 proteins ELK014 96-well kit
Test kits for measuring Total STAT5 proteins ELK017 96-well kit
Test kits for measuring phospho-AKT 1/2/3 (S473)
and Total AKT 1/2/3
ELK003 96-well kit
Test kits for measuring phospho-ERK 1/2 (T202/Y204)
and Total ERK 1/2
ELK006 96-well kit
Test kits for measuring phospho- NF-κB p65 (S536)
and Total NF-κB p65
ELK009 96-well kit
Test kits for measuring phospho-SMAD1 (S463/465)
and Total SMAD1
ELK012 96-well kit
Test kits for measuring phospho-STAT3 (Y705)
and Total STAT3
ELK015 96-well kit
Test kits for measuring phospho-STAT5 A/B (Y694/699)
and Total STAT5 A/B
ELK018 96-well kit

Additional products for individual sale

Companion Products Catalog Number Description
5 x 96-strip-well microplate 6300021 Assay Plate
25 x 96-strip-well microplate 6300023 Assay Plate
120 x 96-strip-well microplates 6300006 Assay Plate
100 mL 5X Lysis Buffer EBB003 5X Lysis Buffer
100 mL Enhancer Solution EBB002 Enhancer Solution
500 mL 10X Wash Buffer EBB001 10X Wash Buffer