Scientific publications

The AlphaScreen® SureFire® technology has been used extensively to interrogate cellular signalling mechanisms for academic and drug discovery applications. Click on the links below to see how scientists are using this technology.


TGR staff also actively contribute to research interrogating kinase signalling mechanisms in areas such as GPCR biology and cancer research. Development and validation of cellular models for cellular signalling studies is a key area of interest.

Sheehan AJ, Goodrich W, Banks P, Crouch MF, Osmond RIW. Development and validation of a single-well cell-based assay for the detection of endogenous phosphoproteins. ASSAY and Drug Development Technologies (2012). In Press. Read more.

Osmond RIW, Martin-Harris MH, Crouch MF, Park J, Morreale E, Dupriez VJ. G-Protein-Coupled Receptor-Mediated MAPK and PI3-Kinase Signaling Is Maintained in Chinese Hamster Ovary Cells after γ-Irradiation. J. Biomol. Screen. 2012, 17(3), 361-369. Read more.

Osmond RIW, Crouch MF, Dupriez V (2010) An emerging role for kinase screening in GPCR drug discovery. Curr Opin Mol Ther. 12(3), 305-15.Read more

Osmond RIW, Das S, Crouch MF. (2010) Development of cell based assays for cytokine receptor signaling, using an AlphaScreen SureFire assay format. Anal. Biochem.403(1–2), 94–101. Read more

Werry TD, Stewart GD, Crouch MF, Watts A, Sexton PM, Christopoulos A. (2008) Pharmacology of 5HT(2C) receptor-mediated ERK1/2 phosphorylation: agonist-specific activation pathways and the impact of RNA editing. Biochem. Pharmacol. 76, 1276-1287. Read more

Crouch MF, Osmond RIW (2008) New strategies in drug discovery for GPCRs: high throughput detection of cellular ERK phosphorylation. Comb Chem High Throughput Screen. 11, 344-356. Read more

Lee HJ, Mun HC, Lewis NC, Crouch MF, Culverston EL, Mason RS, Conigrave AD. (2007) Allosteric activation of the extracellular Ca2+-sensing receptor by L-amino acids enhances ERK1/2 phosphorylation. Biochem J. 404, 141-149. Read more

Matthews CA, Shaw JE, Hooper JA, Young IG, Crouch MF, Campbell HD. (2007) Expression and evolution of the mammalian brain gene Ttyh1. J Neurochem. 100, 693-707. Read more

Osmond RIW, Sheehan A, Borowicz R, Barnett E, Harvey G, Turner C, Brown A, Crouch MF, Dyer AR (2005) GPCR screening via ERK 1/2: a novel platform for screening G protein-coupled receptors. J. Biomol. Screen. 10, 730-737. Read more

Last updated: Dec 2012